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CLARITY in lymph tissue
I'm starting a project that will (hopefully) use CLARITY on lymphatic tissue (at this point we are looking at lymph nodes and gut associate lymph tissue). We plan to set up the protocol as per the standard protocol on fluoro-labeled brain tissue then strike out and optimize for lymph tissue once we know we have everything working.
Is anyone aware off the bat of any major modifications we will need to make to the standard CLARITY protocol in order to successfully embed and/or clear the lymph tissue? I am particularly concerned that the reticulin fibers present a fatal issue.
Any insights will be gratefully recieved!
Is anyone aware off the bat of any major modifications we will need to make to the standard CLARITY protocol in order to successfully embed and/or clear the lymph tissue? I am particularly concerned that the reticulin fibers present a fatal issue.
Any insights will be gratefully recieved!